https://theduongresearchgroup.com/gallery-mojave daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1458581832673-UZTVQK3KYBRBZJEV2TA9/EmptyName-13.jpg Gallery https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1459879490526-G24JE1DJLLKTXZZ7AB3Q/img-01.jpg Gallery https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1461695306540-8QFJK5P595E5ROR2LJ52/img-05.jpg Gallery https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1459879492862-RKJ57EW5U6XYS5SBZ16U/img-08.jpg Gallery https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1461695316996-9T0PB79UV8MG58D1SSJX/img-06.jpg Gallery https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1459879491425-LMMLEQ04TVDPA4ZBY5SJ/img-03.jpg Gallery https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1459879491506-9LEW43M9OMQZEMMON1JW/img-04.jpg Gallery https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1461695299453-AM36QBLDUB7DWF3AIKOH/img-02.jpg Gallery https://theduongresearchgroup.com/home daily 1.0 2024-01-20 https://theduongresearchgroup.com/research daily 0.75 2019-05-05 https://theduongresearchgroup.com/publications daily 0.75 2019-05-05 https://theduongresearchgroup.com/contact-us daily 0.75 2019-05-06 https://theduongresearchgroup.com/team-1 daily 0.75 2019-05-06 https://theduongresearchgroup.com/publications-1 daily 0.75 2025-03-07 https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/4247adcc-85f0-4945-b069-7a6031209697/Untitled+1.jpg Publications Integral membrane proteins (IMPs) are key targets for small-molecule drugs, yet reliable methods to assess their interactions without detergents are lacking. We present the Peptidisc membrane mimetic (MM) to address this. This study combines the Peptidisc method with thermal proteome profiling (TPP) to analyze membrane protein-ligand interactions in a detergent-free setting. Using a library from whole mouse liver, we investigate ATP and orthovanadate's effects on IMP stability, focusing on transporters and receptors. MM-TPP also detects stability changes caused by ATP by-products, confirming non-standard ATP binders through computational methods. This new platform enhances our understanding of ligand effects and membrane protein stability amid small molecule interactions. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117318770-ZKOS4XYBHZS17YB27BSD/Screen+Shot+2019-08-29+at+3.11.51+PM+copy+5.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117422087-MVMKRS5KMPQNG0YSE1EJ/Screen+Shot+2019-08-29+at+3.11.51+PM+copy+6.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567115706262-DNNMDCXJEHVOUVBQHRZW/Screen+Shot+2019-08-29+at+2.34.32+PM.png Publications - Like the conductor of an orchestra … https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1584045895134-5W6KBYANG9DQLXPR17X0/Untitled-1.jpg Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567116953208-YBAG2Y91HBNTZUERW9RZ/Screen+Shot+2019-08-29+at+2.34.32+PM+copy+5.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/0046a9ae-163a-4e2d-b06c-5ca5f7fd237a/1.jpg Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/c5117795-9cb7-4c26-b78b-0d4362aef42d/1.gif Publications - https://pubs.acs.org/doi/10.1021/acs.jproteome.3c00825 https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/df2d973a-159d-4500-bf09-bfcab3a81f7a/2.jpg Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117135338-AQEQ9Q9NFERMQB04EXUZ/Screen+Shot+2019-08-29+at+2.34.32+PM+copy+4.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117199852-ZOIS5KO52CHBKCSINBCD/Screen+Shot+2019-08-29+at+2.45.47+PM+copy+4.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117102477-AURO7HI1K2MWG2FGZ4AH/Screen+Shot+2019-08-29+at+3.11.51+PM.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1602181415721-4VUJQAASNN069YEQEDCO/Screen+Shot+2020-10-07+at+4.43.55+PM.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/cfc07937-1b70-4044-aac8-3bb1c7ba91ac/Fig+5+copy.jpeg Publications - Antony F, Brough Z, Orangi M, Al-Seragi M, Aoki M, Babu M, Duong van Hoa F. Alcohol use and high-fat diets harm liver function. While research has focused on protein changes in alcoholic liver disease (ALD) and metabolic liver disease (MASLD), membrane proteins have been less studied. Traditional methods often miss key membrane details. This study employed the Peptidisc method to analyze liver membrane proteins. In mice on a high-fat diet with ethanol, we identified over 1,500 liver proteins, half of which had transmembrane segments. We found 106 integral membrane proteins altered compared to untreated samples. Gene analysis showed disruptions in lipid metabolism, cell adhesion, foreign substance processing, and mitochondrial membrane formation. Pathways for cholesterol and bile acid transport were affected, suggesting an adaptive response to liver fat buildup. Our research underscores the significance of membrane proteins in disease and highlights potential targets for treatment or diagnosis. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117625021-6L6KY0XADP3Z7YYQVI2J/Screen+Shot+2019-08-29+at+2.34.32+PM+copy.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117265733-EDDFLZQOY71KEKI77A13/Screen+Shot+2019-08-29+at+3.11.51+PM+copy+4.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/24431019-2de5-4e81-9130-57edb47815d4/Untitled+6.jpg Publications - Mohammed Al-Seragi, Yilun Chen, Franck Duong van Hoa. NANOBODIES® (Nbs) are valuable in therapy, diagnostics, and industry due to their small size, enabling them to target areas inaccessible to regular antibodies. While Nbs typically exhibit strong specificity, combining multiple Nbs can enhance binding strength. Recent studies have focused on linking multiple Nb clones, using methods like flexible linkers, antibody domains, self-assembling coils, chemical bonding, and clustering sequences. Successful Nb combinations have been demonstrated in tests and virus neutralization. This review outlines techniques for creating combined Nbs and discusses their uses, benefits, and limitations. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117461438-NPCTKIEPQ5NPMTIKIKXR/Screen+Shot+2019-08-29+at+2.34.32+PM+copy+3.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/03cb98eb-85f0-4de1-8233-b8ac769de319/Untitled+6.jpg Publications - Peptidisc-assisted hydrophobic clustering toward the production of multimeric and multispecific Nanobody proteins. (2025). Biochemistry. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1589656911664-V0PLWDWTLEUQK3H0A8MH/TOC+Revised+copy.jpg Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117530361-TGNZ0415Q6RMS9XJD5KG/Screen+Shot+2019-08-29+at+2.34.32+PM+copy+6.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/9f71605a-87d6-4439-967c-6ec89737c2fc/Figure+1_0203+copy.png Publications - Zora Brough, Zhiyu Zhao and Franck Duong van Hoa Understanding membrane protein expression is vital for cell differentiation, disease characterization, biomarker identification, and therapeutic development. While bottom-up proteomics surveys the membrane proteome, the low abundance and hydrophobicity of these proteins complicate sample preparation. Conventional extraction methods use detergents, which, while preventing aggregation, become contaminants that interfere with analysis. Various detergent removal strategies, including FASP, SP3, S-Trap, and membrane mimetics, exist to address this. This review examines each method's fundamentals, applications, advantages, and limitations, offering insights into their effectiveness in membrane protein research. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/94d4f633-f414-42ef-adb3-b3e4f8ed5c6f/4.png Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117664721-OUI1CIB0F3IV2Q8WEB7S/Screen+Shot+2019-08-29+at+2.28.55+PM.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/33715059-c66e-4a00-88cb-f125bdc2d67b/3.jpg Publications - Make it stand out Whatever it is, the way you tell your story online can make all the difference. https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567116808192-958ZU6JX2A3CGID32VQW/Screen+Shot+2019-08-29+at+3.11.51+PM.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567117576038-89VS67NFLN96AJ41P9Q5/Screen+Shot+2019-08-29+at+2.34.32+PM+copy+2.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1567116925532-POWKG9U6LEJMDNSCJPDY/Screen+Shot+2019-08-29+at+2.34.32+PM+copy+7.png Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/1578508098557-XW0658D8R2BKXM09QZWN/thumbnail_PeptiQuick+Cover+Photo.jpg Publications https://images.squarespace-cdn.com/content/v1/5ccf42b3dddb6cd3625c6b4b/02fbb593-370c-4486-8069-94ea6cf88068/Presentation9.png Publications - Jandu RS, Yu H, Zhao Z, Le HT, Kim S, Huan T and Duong van Hoa F. Protein-lipid interactions are less understood than protein-protein and protein-nucleic acid interactions, primarily because membrane proteins (MPs) are insoluble in water. Detergents used for solubility can disrupt MPs and extract vital lipids. Recently developed membrane mimetics, like peptidisc, permit the isolation of MPs under various lipid conditions, influencing their activity and stability. Peptidisc also enables the incorporation of specific lipids to modify the protein environment and study related interactions. This research offers early insights into protein-lipid interactions, paving the way for further exploration of lipid influences on membrane protein functions. https://theduongresearchgroup.com/about-quote-mojave daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1462307270587-FP4LH74IUETV5IM696NX/img-02.jpg Quote https://theduongresearchgroup.com/about-join-mojave daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1459870151243-UM5MND5R89JE6VXSI8T1/img-07.jpg Join https://theduongresearchgroup.com/our-mission-and-goals-mojave daily 0.75 2025-05-12 https://theduongresearchgroup.com/impact-quote-mojave daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1459879174445-MIOY5PULB1XAW864NVA8/img-06.jpg Quote https://theduongresearchgroup.com/steps-mojave daily 0.75 2025-05-12 https://theduongresearchgroup.com/your-money-mojave daily 0.75 2025-05-12 https://theduongresearchgroup.com/impact-join-mojave daily 0.75 2025-05-12 https://images.squarespace-cdn.com/content/v1/56e049e08a65e26059119cd5/1459870151243-UM5MND5R89JE6VXSI8T1/img-07.jpg Join